A Simple and Fast Method to Sequence the Full-Length Spike Gene for SARS-CoV-2 Variant Identification from Patient Samples

Since the beginning of pandemic, a race has been underway to detect SARS-CoV-2 virus infection (PCR screening, serological diagnostic kits), treat patients (drug repurposing, standard care) and develop vaccine. After almost year active circulation worldwide, variants have appeared in different countries. Those include mutations multiple regions genome, particularly spike gene. Because this surface protein is key player both spread efficacy vaccine strategies, challenge efficiently monitor appearance population. The present work describes procedure based on widely available Sanger technology produce full-length sequence gene from patient-derived samples.